Proteomics and Metabolomics Platform

Overview of Services

Monash University Malaysia's Proteomics and Metabolomics Platform (MUM-PMP) is an advanced research facility dedicated to studying proteins and metabolites
in all biological systems. Equipped with state-of-the-art instrumentation and cutting-edge technologies, the platform enables scientists to delve into the intricacies of
cellular processes and disease mechanisms. The Agilent Integrated Biology Facility @ Monash Malaysia (AIBF) was established to complement and enhance the analytical
capabilities of the Proteomics and Metabolomics Platform.
We aim to provide complete mass spectrometry research services in proteomics and small molecules analysis that supports multidisciplinary research activities for Monash
and other local and international institutions. Our services:
1. Protein Profiling (Proteomics)
2. Peptide Denovo Sequencing
3. Intact Protein/Peptide Mass Determination
4. Small Molecule Profiling (Metabolomics)

Leadership

Location and hours of operation

Links and Resources

1. https://www.monash.edu.my/lcms

Equipment

The Agilent 6550 iFunnel Quadrupole Time-of-Flight (Q-TOF) mass spectrometer delivers the lowest detection levels of any high resolution LC/MS instrument. By incorporating with Agilent Jet Stream technology and dual-stage ion funnel, the system is able to achieve a 10-fold increase in sensitivity for selected molecules as compared to an earlier generation Q-TOF. In combination with ChipCube interface, the robust Agilent 6550 i-Funnel Q-TOF system is specifically set to processed protein/peptide samples derived from various backgrounds such as plant, snake venom, microorganisms which includes viruses, bacteria and fungi, blood plasma and serum and so forth, yet still manage to give reproducible results. Despite being set to process protein/peptide samples, the system is configuration is interchangeable according to the desired setup for analysing various types of samples in order to meet the ever increasing demands from clients.

The Agilent 6520 Q-TOF delivers exceptional analytical performance for MS and MS/MS analyses for experiments demanding the highest accurate-mass measurements. These improvements enable scientists in metabolomics, proteomics and complex qualitative analyses to find and identify more compounds faster than ever. All of this performance is achieved without significantly compromising rapid analysis time for higher-quality data. The Agilent 6520 Q-TOF features attomole sensitivity, better than 2-ppm MS mass accuracy approaching FTMS mass accuracy, better than 5-ppm MS/MS mass accuracy -much better than FTMS mass accuracy at fast spectral acquisition rate, tenfold improvement in in-spectra dynamic range without loss of sensitivity, mass resolution to 20,000 not dependent on spectral acquisition rate, fast data acquisition compatible with fast LC chromatography and broad mass range from m/z 50 to 3200. Although it can carry out other type of analysis, this system is mainly specialised in compound discovery.

Contacts